disc electrophoresis

简明释义

圆盘电泳

英英释义

例句

1.We need to optimize the buffer conditions for disc electrophoresis 盘电泳 to improve resolution.

我们需要优化盘电泳 disc electrophoresis的缓冲液条件以提高分辨率。

2.In our lab, we often use disc electrophoresis 盘电泳 to separate proteins based on their size.

在我们的实验室，我们经常使用盘电泳 disc electrophoresis 根据蛋白质的大小进行分离。

3.The disc electrophoresis 盘电泳 technique is widely used in genetic analysis.

这种盘电泳 disc electrophoresis技术广泛应用于基因分析。

4.The results of the disc electrophoresis 盘电泳 showed distinct bands for each sample.

这次盘电泳 disc electrophoresis的结果显示每个样品都有明显的条带。

5.During the experiment, we observed unexpected migration patterns in disc electrophoresis 盘电泳.

在实验过程中，我们观察到盘电泳 disc electrophoresis中出现了意外的迁移模式。

作文

Electrophoresis is a vital technique in molecular biology and biochemistry that allows scientists to separate and analyze macromolecules such as proteins and nucleic acids. One specific type of this technique is known as disc electrophoresis（圆盘电泳）. This method has gained significant importance due to its efficiency and effectiveness in separating various biomolecules based on their size and charge. In this essay, I will discuss the principles behind disc electrophoresis（圆盘电泳）, its applications, and its advantages over other methods of electrophoresis.The fundamental principle of disc electrophoresis（圆盘电泳）lies in the movement of charged particles in an electric field. When a mixture of molecules is subjected to an electric field, the charged molecules migrate towards the electrode with the opposite charge. The rate of migration depends on several factors, including the size of the molecule, its charge, and the medium through which it is moving. In disc electrophoresis（圆盘电泳）, the gel matrix is typically composed of polyacrylamide, which provides a porous environment for the separation process.One of the key features of disc electrophoresis（圆盘电泳） is the use of a discontinuous gel system. This means that the gel consists of two layers: a stacking gel and a resolving gel. The stacking gel has a lower concentration of acrylamide, which allows larger molecules to concentrate into narrow bands before entering the resolving gel, where they are separated based on their size. This unique setup enhances the resolution of the separation process, making it possible to distinguish between closely related molecules.The applications of disc electrophoresis（圆盘电泳） are vast and varied. In the field of protein analysis, this technique is extensively used to study protein purity, molecular weight, and enzyme activity. Researchers often employ disc electrophoresis（圆盘电泳） to analyze the composition of complex mixtures, such as those found in biological samples. Additionally, it plays a crucial role in the identification of genetic markers and the study of DNA fragments in molecular genetics.Another significant advantage of disc electrophoresis（圆盘电泳） is its ability to provide rapid results. The process can be completed within a few hours, allowing researchers to obtain valuable data quickly. Furthermore, the method is relatively straightforward and can be performed with standard laboratory equipment, making it accessible for many research institutions.Moreover, disc electrophoresis（圆盘电泳） is also beneficial in clinical diagnostics. It is commonly used to analyze serum proteins in patients, aiding in the diagnosis of various diseases, including multiple myeloma and liver disorders. By comparing the protein profiles obtained from patient samples to established reference patterns, clinicians can make informed decisions regarding patient care.In conclusion, disc electrophoresis（圆盘电泳） is an essential tool in the fields of biochemistry and molecular biology. Its ability to separate and analyze macromolecules efficiently makes it invaluable for both research and clinical applications. As technology advances, we can expect further improvements in the techniques and methodologies surrounding disc electrophoresis（圆盘电泳）, leading to even greater insights into the molecular world around us. Understanding and mastering this technique is crucial for anyone involved in scientific research, as it opens doors to new discoveries and innovations in the life sciences.

电泳是一种在分子生物学和生物化学中至关重要的技术，它允许科学家分离和分析大分子，如蛋白质和核酸。这种技术的一种特定类型被称为圆盘电泳。由于其在根据大小和电荷分离各种生物分子方面的高效性和有效性，这种方法变得越来越重要。在这篇文章中，我将讨论圆盘电泳的原理，它的应用以及它相对于其他电泳方法的优势。圆盘电泳的基本原理在于带电粒子在电场中的移动。当一混合物受到电场的影响时，带电分子会朝着与其带有相反电荷的电极迁移。迁移速率取决于多个因素，包括分子的大小、其电荷以及其移动的介质。在圆盘电泳中，凝胶基质通常由聚丙烯酰胺组成，这为分离过程提供了多孔环境。圆盘电泳的一大关键特征是使用不连续凝胶系统。这意味着凝胶由两层组成：堆叠凝胶和分辨凝胶。堆叠凝胶的聚丙烯酰胺浓度较低，允许较大的分子在进入分辨凝胶之前浓缩成窄带，在分辨凝胶中，它们根据大小进行分离。这种独特的设置增强了分离过程的分辨率，使得可以区分密切相关的分子。圆盘电泳的应用广泛而多样。在蛋白质分析领域，这种技术被广泛用于研究蛋白质纯度、分子量和酶活性。研究人员经常利用圆盘电泳来分析复杂混合物的组成，例如在生物样本中发现的那些。此外，它在遗传标记的识别和分子遗传学中DNA片段的研究中也发挥着关键作用。圆盘电泳的另一个显著优势是其快速结果的能力。该过程可以在几个小时内完成，使研究人员能够迅速获得有价值的数据。此外，该方法相对简单，可以使用标准实验室设备进行操作，使许多研究机构都能接触到它。此外，圆盘电泳在临床诊断中也很有益。它通常用于分析患者的血清蛋白，帮助诊断各种疾病，包括多发性骨髓瘤和肝脏疾病。通过将从患者样本中获得的蛋白质谱与已建立的参考模式进行比较，临床医生可以做出关于患者护理的明智决策。总之，圆盘电泳是生物化学和分子生物学领域的重要工具。它高效分离和分析大分子的能力使其在研究和临床应用中都不可或缺。随着技术的进步，我们可以期待围绕圆盘电泳的技术和方法论的进一步改进，从而为我们提供更深入的分子世界洞察。理解和掌握这一技术对于任何参与科学研究的人来说都是至关重要的，因为它为生命科学的新发现和创新打开了大门。