zone electrophoresis

简明释义

区域电泳

英英释义

例句

1.In the laboratory, we conducted a test using zone electrophoresis 区带电泳 to separate the proteins from the cell extracts.

在实验室，我们使用区带电泳 zone electrophoresis 来分离细胞提取物中的蛋白质。

2.After performing zone electrophoresis 区带电泳, we analyzed the migration patterns of the samples.

在进行完区带电泳 zone electrophoresis后，我们分析了样品的迁移模式。

3.One common application of zone electrophoresis 区带电泳 is in the diagnosis of genetic disorders.

一种常见的区带电泳 zone electrophoresis 应用是在遗传疾病的诊断中。

4.The results of the zone electrophoresis 区带电泳 showed distinct bands for each type of DNA fragment.

这次的区带电泳 zone electrophoresis 结果显示每种DNA片段都有明显的条带。

5.Researchers often prefer zone electrophoresis 区带电泳 due to its high resolution in separating biomolecules.

研究人员通常更喜欢区带电泳 zone electrophoresis，因为它在分离生物分子方面具有高分辨率。

作文

Electrophoresis is a fundamental technique used in biochemistry and molecular biology to separate charged particles, such as proteins or nucleic acids, based on their size and charge. One specific type of electrophoresis is known as zone electrophoresis, which plays a crucial role in the analysis of biological samples. In zone electrophoresis (区带电泳), the sample is applied to a gel matrix, and when an electric field is applied, the charged molecules migrate through the gel at different rates. This separation occurs because smaller molecules can move more easily through the pores of the gel than larger ones, leading to distinct zones of separation. The principle behind zone electrophoresis is relatively straightforward. When an electric current is introduced, negatively charged molecules move towards the positive electrode, while positively charged molecules migrate towards the negative electrode. The result is a distinct separation of different components within the sample, which can then be visualized using various staining techniques. This method has been widely adopted for analyzing proteins, DNA fragments, and RNA, making it an invaluable tool in both research and clinical laboratories.One of the key advantages of zone electrophoresis is its ability to provide high-resolution separations. This means that even closely related molecules can be distinguished from one another, which is particularly important in applications such as genetic testing or protein characterization. For instance, in the field of genetics, researchers can use zone electrophoresis to analyze DNA fragments generated by restriction enzymes, allowing them to identify genetic variations or mutations. Similarly, in proteomics, this technique can help in the identification of specific proteins within complex mixtures.Another significant aspect of zone electrophoresis is its versatility. It can be performed using various types of gels, including agarose and polyacrylamide, depending on the size of the molecules being analyzed. Agarose gel is commonly used for separating larger DNA fragments, while polyacrylamide gel is preferred for smaller proteins or peptides. Additionally, modifications to the traditional zone electrophoresis setup can enhance its effectiveness. For example, incorporating gradient gels can improve resolution, and using different buffer systems can optimize the separation of specific molecules.Despite its many advantages, zone electrophoresis also has limitations. One challenge is that the separation process can be influenced by factors such as the concentration of the gel, the voltage applied, and the ionic strength of the buffer. These variables must be carefully controlled to achieve optimal results. Furthermore, while zone electrophoresis is excellent for separating molecules, it does not provide information about the functional properties of the separated components. Therefore, it is often used in conjunction with other techniques, such as mass spectrometry or western blotting, to gain a comprehensive understanding of the samples being analyzed.In conclusion, zone electrophoresis (区带电泳) is a powerful and essential technique in the fields of biochemistry and molecular biology. Its ability to separate charged molecules based on size and charge makes it invaluable for a wide range of applications, from genetic analysis to protein characterization. As research continues to advance, the methods and applications of zone electrophoresis are likely to evolve, further enhancing its utility in scientific discovery and clinical diagnostics.