refolding

简明释义

英[ˌriːˈfoʊldɪŋ]美[ˌriːˈfoʊldɪŋ]

n. [地质]重褶皱作用；[生化]重折叠

v. 再次折叠（refold 的 ing 形式）

英英释义

单词用法

同义词

反义词

例句

1.The recent research progress of recombinant protein refolding was reviewed.

本文对近年来重组蛋白复性技术的研究进行了评述。

2.Protein refolding by dilution is the basis of research on other method of renaturation.

稀释复性法是研究其他方法的基础。

3.Most of the proteins examined so far proceed oxidative refolding via multiple pathways rather than a single and specific pathway. This is consistent with the folding energy landscape theory.

在已研究过的蛋白质中，大多数蛋白质都是沿着多途径而非单一、特定的途径进行氧化重折叠，这与折叠能量景观学说是一致的。

4.In this chapter solute retention mechanism in LC, protein refolding mechanism and new developments in protein refolding kinetics were reviewed. It contains 61 references.

本章对色谱中的保留机理及理论、蛋白质液相色谱复性机理及蛋白折叠动力学理论模型研究进展进行了综述，共包括文献61篇。

5.Objective The possibility of peripherin binding protein (PBP) to improve the refolding of denatured luciferase in vitro was investigated.

目的探讨感光受体外周蛋白结合蛋白（PBP）在体外是否有促进变性蛋白质复性的作用。

6.Recently the refolding studies have been concentrated on alkaline phosphatase from E.

对小牛肠碱性磷酸酶再折叠的机制及其影响因素进行了探讨。

7.The origami expert demonstrated the technique of refolding to create a more complex design.

折纸专家展示了重新折叠的技巧，以创造出更复杂的设计。

8.After washing the shirt, I spent some time refolding it to make it neat again.

洗完衬衫后，我花了一些时间重新折叠它，使其再次整齐。

9.After the meeting, I had to refold the presentation materials for the next session.

会议结束后，我不得不重新折叠演示材料，以便下一个环节使用。

10.In the lab, scientists often focus on refolding proteins to restore their functional shape.

在实验室，科学家们经常专注于重新折叠蛋白质，以恢复其功能形状。

11.She was refolding the map to fit it back into the glove compartment.

她正在重新折叠地图，以便将其放回手套箱。

作文

In the realm of molecular biology, the process of protein synthesis is intricate and essential for life. Proteins are formed by long chains of amino acids that fold into specific three-dimensional shapes, which determine their function. However, sometimes these proteins do not fold correctly, leading to malfunction or diseases. This is where the concept of refolding comes into play. Refolding refers to the process of restoring a misfolded protein to its correct structure, allowing it to function properly once again. Understanding this process is crucial for various applications in biotechnology and medicine.When proteins are synthesized in the cell, they often begin as linear chains. The folding occurs spontaneously but can be influenced by several factors, including temperature, pH, and the presence of other molecules. If the environment is not optimal, the protein may end up in a misfolded state. Such misfolded proteins can aggregate, leading to diseases such as Alzheimer's or Parkinson's. Therefore, the ability to induce refolding is not just a scientific curiosity; it has significant implications for health and disease management.Researchers have developed various methods to promote refolding. These methods typically involve adjusting the conditions under which the protein is allowed to fold. For example, a common technique is to change the concentration of salts or detergents in the solution, which can help stabilize the correct folding pathway. Additionally, chaperone proteins, which assist in the folding process, can be introduced to facilitate refolding. These chaperones bind to the misfolded proteins and guide them through the correct folding pathways, ensuring that they achieve their functional conformation.The significance of refolding extends beyond basic research. In the field of biotechnology, many therapeutic proteins are produced using recombinant DNA technology. These proteins need to be properly folded to be effective. If they are misfolded during production, they may not be able to bind to their target receptors or catalyze reactions effectively. Thus, optimizing the refolding process is vital for the production of safe and effective biopharmaceuticals.Moreover, the study of refolding processes has led to advancements in drug development. By understanding how proteins misfold and how they can be refolded, scientists can design small molecules that aid in the refolding process or prevent misfolding altogether. Such drugs could potentially treat diseases caused by protein misfolding, offering hope for patients suffering from currently incurable conditions.In summary, refolding is a fundamental concept in protein chemistry that holds great promise for both scientific research and practical applications. As we continue to explore the mechanisms behind protein folding and refolding, we pave the way for innovative treatments and advancements in biotechnology. Understanding how to manipulate these processes will not only enhance our knowledge of cellular functions but also provide solutions to some of the most pressing health challenges of our time.

在分子生物学领域，蛋白质合成的过程复杂而重要，对于生命至关重要。蛋白质由长链氨基酸组成，这些氨基酸折叠成特定的三维形状，决定了它们的功能。然而，有时这些蛋白质并没有正确折叠，导致功能失常或疾病。这就是refolding这个概念发挥作用的地方。refolding指的是将错误折叠的蛋白质恢复到其正确结构的过程，使其能够再次正常工作。理解这一过程对于生物技术和医学的各种应用至关重要。当蛋白质在细胞中合成时，它们通常以线性链的形式开始。折叠是自发发生的，但可以受到多种因素的影响，包括温度、pH值以及其他分子的存在。如果环境不理想，蛋白质可能最终处于错误折叠状态。这种错误折叠的蛋白质可能会聚集，导致阿尔茨海默病或帕金森病等疾病。因此，诱导refolding的能力不仅是科学上的好奇心；它对健康和疾病管理具有重要意义。研究人员开发了多种促进refolding的方法。这些方法通常涉及调整蛋白质允许折叠的条件。例如，一种常见的技术是改变溶液中盐或洗涤剂的浓度，这可以帮助稳定正确的折叠路径。此外，可以引入伴侣蛋白，这些蛋白质在折叠过程中提供帮助，以促进refolding。这些伴侣蛋白与错误折叠的蛋白质结合，并指导它们通过正确的折叠路径，确保它们达到功能构象。refolding的重要性超越了基础研究。在生物技术领域，许多治疗性蛋白质是通过重组DNA技术生产的。这些蛋白质需要正确折叠才能有效。如果它们在生产过程中错误折叠，可能无法有效地与其目标受体结合或催化反应。因此，优化refolding过程对于安全有效的生物制药生产至关重要。此外，refolding过程的研究推动了药物开发的进步。通过理解蛋白质如何错误折叠以及如何进行refolding，科学家可以设计小分子，帮助refolding过程或防止错误折叠。这类药物有潜力治疗由蛋白质错误折叠引起的疾病，为当前无法治愈的患者带来希望。总之，refolding是蛋白质化学中的一个基本概念，具有巨大的科学研究和实际应用前景。随着我们继续探索蛋白质折叠和refolding过程背后的机制，我们为生物技术的创新治疗和进步铺平了道路。理解如何操控这些过程不仅会增强我们对细胞功能的认识，还将为解决我们时代一些最紧迫的健康挑战提供解决方案。