immunoblotting

简明释义

英[ɪˈmjunəˌblɒtɪŋ]美[ɪˈmjunəˌblɑtɪŋ]

[免疫] 免疫印迹法

英英释义

单词用法

同义词

反义词

例句

1.The tyrosine phosphorylation of IRS-1 was measured by immunoprecipitation and enhanced chemiluminescent immunoblotting technique.

采用免疫沉淀及增强化学发光法检测IRS 1蛋白质酪氨酸磷酸化；

2.Immunoblotting analysis demonstrated that the exoantigens were more suitable for being used as an indicator for taxonomy because the specificity of antisera to exoantigens was better tha.

免疫印渍分析显示，体外抗原更适于用作免疫分类鉴定的指标，因为用体外抗原免疫动物所产生的抗体的特异性比菌丝体可溶性蛋白质要好。

3.Immunoblotting analysis demonstrated that the exoantigens were more suitable for being used as an indicator for taxonomy because the specificity of antisera to exoantigens was better tha.

免疫印渍分析显示，体外抗原更适于用作免疫分类鉴定的指标，因为用体外抗原免疫动物所产生的抗体的特异性比菌丝体可溶性蛋白质要好。

4.Semiquantitation of HSPs was performed by immunoblotting.

热休克蛋白半定量是执行的免疫。

5.Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques.

附加表位对于用免疫印迹、免疫沉淀和免疫染色技术来进行蛋白质的标记和检测很有用。

6.The binding ability of these deletant proteins to AD5-10, a mouse anti-human DR5 monoclonal antibody, was evaluated by immunoblotting and ELISA.

利用蛋白免疫印迹及ELISA技术研究重组蛋白与小鼠抗人DR5单克隆抗体AD5-10的结合情况。

7.The reactions of sera from patients with liver diseases to the antigenic polypeptides of liver specific membrane lipoproteins (LSP) were studied with the immunoblotting technique.

本文采用免疫转印技术分析了肝病患者血清与肝特异性膜脂蛋白（LSP）抗原性多肽成份的反应。

8.By way of immunoprecipitation and immunoblotting, the cytosolic protein tyrosine phosphorylation was examined.

用免疫沉淀和免疫印迹法测定胞浆蛋白酪氨酸磷酸化情况。

9.Use as a blocking reagent to evaluate the specificity of antibody reactivity in western immunoblotting and Immunohistochemistry protocols.

本品作为阻断剂，用于检测抗体在免疫印迹和免疫组织化学检测中的特异性。

10.Objective To compare the difference of enzyme-linked immunosorbent assay(ELISA), Radioimmunoassay(RIA) and immunoblotting(IB) in the determination of insulin autoantibody(IAA).

目的比较酶联免疫（ELISA）、放射免疫（RIA）和免疫印迹（IB）法检测胰岛素自身抗体（IAA）敏感性和特异性的差别。

11.After performing immunoblotting, we found an increased expression of the target protein.

在进行免疫印迹后，我们发现目标蛋白的表达增加。

12.The researchers used immunoblotting to detect specific proteins in the cell lysates.

研究人员使用免疫印迹技术检测细胞裂解液中的特定蛋白质。

13.In this experiment, immunoblotting was crucial for validating the presence of the antibody.

在这个实验中，免疫印迹对于验证抗体的存在至关重要。

14.We utilized immunoblotting to analyze the phosphorylation status of the enzyme.

我们利用免疫印迹分析酶的磷酸化状态。

15.The results from the immunoblotting confirmed our hypothesis about protein interactions.

来自免疫印迹的结果证实了我们关于蛋白质相互作用的假设。

作文

In the field of molecular biology, techniques that allow scientists to study proteins are crucial for understanding cellular processes. One such technique is immunoblotting, which is commonly referred to as Western blotting. This method is used to detect specific proteins in a sample through the use of antibodies. The significance of immunoblotting lies in its ability to provide both qualitative and quantitative data about protein expression levels, making it an indispensable tool in research and diagnostics.The process of immunoblotting begins with the separation of proteins by gel electrophoresis. This technique allows proteins to be sorted based on their size and charge. Once separated, the proteins are transferred from the gel onto a membrane, typically made of nitrocellulose or PVDF. This transfer is critical as it preserves the spatial arrangement of the proteins, allowing for precise identification later in the process.After the transfer, the membrane is incubated with a blocking solution to prevent non-specific binding of antibodies. This step is essential to enhance the specificity of the subsequent antibody interactions. Following blocking, the membrane is treated with primary antibodies that specifically bind to the target protein of interest. These antibodies are usually raised in animals, such as rabbits or mice, and are designed to recognize unique epitopes on the protein.Once the target proteins are bound by the primary antibodies, the membrane is washed to remove any unbound antibodies. Subsequently, a secondary antibody, which is conjugated to a detectable marker (such as an enzyme or fluorescent dye), is applied. This secondary antibody binds to the primary antibody, amplifying the signal and enabling visualization of the target protein.The final step in immunoblotting is the detection of the protein-antibody complex. Depending on the type of secondary antibody used, various detection methods can be employed. For instance, chemiluminescent substrates can be added, producing light in the presence of the enzyme linked to the secondary antibody, which can then be captured on photographic film or digital imaging systems. Alternatively, fluorescent detection can be used, allowing for real-time monitoring of protein levels.The applications of immunoblotting are vast and varied. In clinical settings, it is often used to diagnose diseases, including infections and autoimmune disorders, by detecting specific biomarkers. Research laboratories utilize this technique to study protein expression in different conditions, such as cancer, where abnormal protein levels can indicate disease progression or response to treatment.Moreover, immunoblotting has been pivotal in the development of therapeutic antibodies. By understanding how certain proteins behave under various conditions, researchers can design better-targeted therapies for diseases. This technique also plays a crucial role in vaccine development, where it is used to confirm the presence of specific antigens in vaccine candidates.In conclusion, immunoblotting is a fundamental technique in the life sciences that enables researchers to analyze proteins with high specificity and sensitivity. Its versatility and reliability make it a cornerstone method for both basic research and clinical diagnostics. As our understanding of proteomics expands, the importance of immunoblotting will undoubtedly continue to grow, providing insights into the complex world of proteins and their functions within biological systems.

在分子生物学领域，允许科学家研究蛋白质的技术对于理解细胞过程至关重要。其中一种技术是免疫印迹法，通常称为西方印迹法。该方法用于通过抗体检测样本中的特定蛋白质。免疫印迹法的重要性在于其能够提供关于蛋白质表达水平的定性和定量数据，使其成为研究和诊断中不可或缺的工具。免疫印迹法的过程始于通过凝胶电泳分离蛋白质。这种技术允许根据蛋白质的大小和电荷对其进行排序。分离后，蛋白质从凝胶转移到膜上，通常由硝酸纤维素或PVDF制成。此转移至关重要，因为它保留了蛋白质的空间排列，从而允许在后续过程中精确识别。转移后，膜被孵育于封闭溶液中，以防止抗体的非特异性结合。这一步骤对于增强后续抗体相互作用的特异性至关重要。封闭后，膜被处理以加入特定结合目标蛋白的初级抗体。这些抗体通常是在动物（如兔子或小鼠）体内产生的，旨在识别蛋白质上的独特表位。一旦目标蛋白与初级抗体结合，膜就会被洗涤以去除未结合的抗体。随后，应用与可检测标记（如酶或荧光染料）结合的次级抗体。这个次级抗体与初级抗体结合，放大信号并使目标蛋白可视化。免疫印迹法的最后一步是检测蛋白质-抗体复合物。根据所使用的次级抗体类型，可以采用多种检测方法。例如，可以添加化学发光底物，在与次级抗体连接的酶存在时产生光，然后可以在照相胶卷或数字成像系统上捕获。或者，可以使用荧光检测，允许实时监测蛋白质水平。免疫印迹法的应用广泛而多样。在临床环境中，它通常用于通过检测特定生物标志物来诊断疾病，包括感染和自身免疫性疾病。研究实验室利用该技术研究不同条件下的蛋白质表达，例如癌症，其中异常的蛋白质水平可能表明疾病进展或对治疗的反应。此外，免疫印迹法在治疗抗体的开发中发挥了关键作用。通过了解特定蛋白质在各种条件下的行为，研究人员可以设计出更好的靶向治疗方案。该技术在疫苗开发中也起着至关重要的作用，用于确认疫苗候选物中是否存在特定抗原。总之，免疫印迹法是生命科学中的一项基本技术，使研究人员能够以高特异性和灵敏度分析蛋白质。其多功能性和可靠性使其成为基础研究和临床诊断的基石方法。随着我们对蛋白质组学的理解不断深入，免疫印迹法的重要性无疑将继续增长，为我们提供对蛋白质及其在生物系统中功能的复杂世界的洞察。