subculturing

简明释义

英[ˈsʌbkʌltʃərɪŋ]美[ˈsʌbkʌltʃərɪŋ]

n. 继代培养

英英释义

单词用法

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例句

1.The shapes of human embryonic lung fibroblasts which were isolated by enzyme digestion were differ, and the culture was all composed of fibroblasts after subculturing for 4-5 generations.

而消化的人胚肺组织中细胞形态不一，经继续培养4～5代后，培养物完全由成纤维细胞构成。

2.Sectoring became visible after subculturing for 5-6 generations, more frequently with 13s8.

菌株角变现象在继代培养5 - 6代后表现明显，而13s8角变子出现的频更高。

3.The shapes of human embryonic lung fibroblasts which were isolated by enzyme digestion were differ, and the culture was all composed of fibroblasts after subculturing for 4-5 generations.

而消化的人胚肺组织中细胞形态不一，经继续培养4～5代后，培养物完全由成纤维细胞构成。

4.Suspension single cells were obtained after successive subculturing of 3 or 4 generations.

经3～ 4次继代培养即可得到悬浮的单细胞。

5.In microbiology, subculturing is essential for maintaining the viability of bacterial strains.

在微生物学中，分培养 对于维持细菌株的活性至关重要。

6.The lab technician performed subculturing to isolate pure colonies from the mixed culture.

实验室技术员进行了分培养，以从混合培养中分离纯菌落。

7.After a week of growth, the scientist began subculturing the cells to new flasks.

经过一周的生长，科学家开始将细胞进行分培养到新的瓶子中。

8.Proper techniques in subculturing can prevent contamination in cell cultures.

在细胞培养中，正确的分培养技术可以防止污染。

9.The process of subculturing allows researchers to expand their samples for further experiments.

进行分培养的过程使研究人员能够扩大样本以进行进一步实验。

作文

In the field of microbiology, the term subculturing refers to the process of transferring microorganisms from one culture medium to another, allowing for the growth and maintenance of specific strains or species. This technique is essential for researchers and scientists who seek to study the characteristics, behaviors, and interactions of various microorganisms. By performing subculturing (亚培养), scientists can ensure that they have a pure and viable culture for experimentation and analysis.The importance of subculturing cannot be overstated. It plays a critical role in various applications, including medical research, environmental studies, and industrial microbiology. For instance, in a laboratory setting, if a researcher isolates a particular bacterium from a sample, they will often use subculturing to create a pure culture of that bacterium. This allows them to conduct experiments without the interference of other microorganisms that may be present in the original sample.Moreover, subculturing is also vital for preserving microbial strains. Many microorganisms have specific growth requirements and may not survive indefinitely in their original culture conditions. By periodically subculturing (亚培养) these microorganisms onto fresh media, researchers can extend their viability and maintain their genetic and phenotypic characteristics for future studies.In addition to its practical applications, subculturing is also a fascinating process that highlights the adaptability and resilience of microorganisms. For example, when transferring bacteria to new media, scientists often observe changes in growth patterns, metabolic activity, and even genetic expression. These observations can provide valuable insights into how microorganisms respond to different environmental conditions and stressors.Furthermore, the technique of subculturing has evolved with advancements in technology. Modern microbiological practices now include automated systems for transferring cultures, which increase efficiency and reduce the risk of contamination. Such innovations have made it easier for researchers to manage large numbers of microbial strains and conduct high-throughput experiments.However, it is crucial to perform subculturing with precision and care. Contamination can easily occur during the transfer process, leading to mixed cultures that can compromise experimental results. Therefore, aseptic techniques and sterile equipment are paramount to successful subculturing (亚培养). Researchers must remain vigilant and adhere to strict protocols to ensure the integrity of their cultures.In conclusion, subculturing is a fundamental technique in microbiology that enables scientists to isolate, maintain, and study microorganisms effectively. Its applications are vast, ranging from fundamental research to industrial processes. As we continue to explore the microscopic world, the role of subculturing (亚培养) will undoubtedly remain a cornerstone of microbiological practices, driving innovation and discovery in the life sciences.