transfection

简明释义

英[trænsˈfekʃən]美[trænsˈfekʃən]

n. 转染

英英释义

单词用法

同义词

反义词

例句

1.Flow cytometry revealed that E1A transfection induced s stage suppression and G2 stage arrest in Hela cells.

流式细胞术结果显示：转染e1 A基因后，细胞周期出现S期抑制，G 2期阻滞。

2.Objective To study the effects of transfection of tyrosine kinases on the tumorigenesis of nasopharyngeal carcinoma.

目的：研究酪氨酸激酶转染对鼻咽癌细胞成瘤的影响。

3.Objective To explore the efficiency of transfection of antisense oligonucleotides HA-2741 by ultrasound mediated liposome microbubbles.

目的探讨超声介导脂质体微气泡转染反义寡核苷酸ha- 2741的有效性。

4.The gene transfection technology might be one of the approaches to resolve these issues.

应用基因转染技术可能是解决这些问题的途径之一。

5.Proliferation suppression and apoptosis of 8348 cells pre and post transfection induced by cisplatin were analysed by MTT methods.

用MTT法分析顺铂对转染前后的8348细胞抑制增殖和诱导凋亡的能力。

6.Gene transfection is the key technique of gene therapy.

基因治疗的关键技术是基因转移。

7.CONCLUSION Lipofectamine may be used as a tool to enhance the efficacy of transfection with recombinant adenovirus.

结论可以利用阳离子脂质体来提高重组腺病毒的转染效率。

8.In this experiment, we optimized the conditions for transfection to maximize gene delivery.

在这个实验中，我们优化了转染的条件以最大化基因传递。

9.Successful transfection can lead to the expression of therapeutic proteins in target cells.

成功的转染可以导致目标细胞中治疗性蛋白的表达。

10.The transfection efficiency was assessed using a fluorescence marker.

使用荧光标记评估了转染效率。

11.The researchers performed transfection to introduce the new gene into the cancer cells.

研究人员进行了转染以将新基因引入癌细胞。

12.We used lipid-based reagents for efficient transfection of our plasmid DNA.

我们使用脂质基试剂高效地进行我们的质粒DNA的转染。

作文

Transfection is a crucial technique in molecular biology that involves the introduction of foreign nucleic acids into cells. This process can be used to study gene function and regulation, develop new therapeutic strategies, and produce recombinant proteins. The ability to manipulate genes at the cellular level has opened up numerous avenues for research and clinical applications. For instance, researchers often use transfection to introduce plasmids containing specific genes into target cells, allowing them to observe the effects of those genes on cell behavior. This is particularly useful in cancer research, where understanding gene expression can lead to better treatment options. There are several methods of transfection, each with its own advantages and disadvantages. Some of the most common techniques include lipofection, electroporation, and viral transduction. Lipofection uses lipid-based reagents to encapsulate nucleic acids, facilitating their entry into cells. This method is relatively easy to use and efficient for many cell types. On the other hand, electroporation involves applying an electrical field to cells, which temporarily disrupts the cell membrane, allowing nucleic acids to enter. While this method can be very effective, it may also cause cell damage if not optimized properly. Viral transduction takes advantage of the natural ability of viruses to infect cells and deliver genetic material. This method is highly efficient but requires careful handling due to the potential risks associated with using viral vectors. The applications of transfection are vast and varied. In the field of gene therapy, for example, scientists are exploring ways to use transfection to deliver therapeutic genes to patients with genetic disorders. By introducing functional copies of genes into affected cells, it may be possible to correct the underlying cause of diseases such as cystic fibrosis or muscular dystrophy. Additionally, transfection plays a significant role in vaccine development. Researchers can use transfected cells to produce antigens that stimulate an immune response, paving the way for new vaccines against infectious diseases. Moreover, transfection is also essential in the production of monoclonal antibodies. By transfecting hybridoma cells with genes encoding specific antibodies, scientists can generate large quantities of these proteins for research and therapeutic purposes. This has been particularly important in the development of targeted therapies for various cancers, where monoclonal antibodies are used to specifically target and kill cancer cells without harming normal tissues. Despite its many benefits, transfection is not without challenges. One of the main issues researchers face is achieving high transfection efficiency while minimizing cytotoxicity. Different cell types respond differently to transfection methods, and optimizing conditions for each specific application can be time-consuming and complex. Furthermore, the stability of the introduced nucleic acids within the cell can also vary, affecting the duration of gene expression. In conclusion, transfection (转染) is an invaluable tool in modern molecular biology that enables scientists to explore gene function, develop new therapies, and produce essential proteins. As technology advances, we can expect to see even more innovative applications of transfection in research and medicine, ultimately leading to improved health outcomes for patients worldwide.