axenic culture

简明释义

纯培养

英英释义

例句

1.In microbiology, researchers often use axenic culture to study the growth of a specific microorganism in isolation.

在微生物学中，研究人员通常使用无菌培养来研究特定微生物在孤立条件下的生长。

2.Aquaculture often employs axenic culture techniques to raise fish free from pathogens.

水产养殖常常采用无菌培养技术来养殖没有病原体的鱼类。

3.Using axenic culture allows scientists to observe the effects of antibiotics on bacteria without interference from other organisms.

使用无菌培养可以让科学家观察抗生素对细菌的影响，而不受其他生物的干扰。

4.The production of vaccines requires axenic culture to ensure that no contaminants affect the final product.

疫苗的生产需要无菌培养以确保最终产品不受污染物的影响。

5.In plant tissue culture, axenic culture is essential for preventing fungal contamination.

在植物组织培养中，无菌培养对于防止真菌污染至关重要。

作文

In the field of microbiology, the concept of axenic culture refers to a method of growing organisms in isolation from any other species. This technique is essential for researchers who want to study the specific characteristics and behaviors of a single type of organism without interference from others. An axenic culture is achieved by ensuring that the growth environment is free from contamination, which can significantly affect the results of experiments. The importance of this method cannot be overstated, as it allows scientists to obtain pure samples that are crucial for accurate analysis.The process of establishing an axenic culture involves several steps. First, researchers must select the organism they wish to study. This could be a bacterium, fungus, or even a plant cell. Once the organism is chosen, the next step is to prepare the growth medium. This medium must be carefully sterilized to eliminate any existing microbes that could contaminate the culture. Techniques such as autoclaving or using chemical disinfectants are commonly employed to achieve sterility.After preparing the medium, the next step is to inoculate it with the selected organism. This is done under sterile conditions, often in a laminar flow hood, to prevent any airborne contaminants from entering the culture. Once the organism is introduced to the medium, it begins to grow and multiply. Throughout this growth period, it is crucial to monitor the culture closely to ensure that no unwanted organisms are present. Any signs of contamination must be addressed immediately, as they can compromise the integrity of the axenic culture.One of the primary advantages of working with an axenic culture is the ability to conduct controlled experiments. For instance, researchers can manipulate environmental factors such as temperature, pH, and nutrient availability to observe how these changes affect the growth and behavior of the organism. This level of control is particularly valuable in fields such as pharmacology, where understanding how a drug interacts with a specific microorganism is essential for developing effective treatments.Moreover, axenic cultures play a vital role in biotechnology and agriculture. In biotechnology, they are used to produce enzymes, antibiotics, and other bioactive compounds. By cultivating microorganisms in isolation, scientists can maximize yield and purity, which are critical for industrial applications. In agriculture, axenic cultures are used to study plant pathogens and develop disease-resistant varieties, thereby improving crop yields and food security.However, despite the numerous benefits of axenic cultures, there are also challenges associated with this technique. Maintaining sterility throughout the entire process can be difficult, and even minor lapses in technique can lead to contamination. Additionally, some organisms are inherently difficult to cultivate in isolation due to their complex relationships with other species. In such cases, researchers may need to explore alternative methods, such as co-culture systems, to better understand these interactions.In conclusion, the practice of creating and maintaining axenic cultures is a cornerstone of microbiological research. It provides scientists with the ability to study organisms in a controlled environment, free from external influences. While there are challenges to overcome, the benefits of axenic cultures far outweigh the drawbacks. As research continues to advance, the techniques surrounding axenic cultures will undoubtedly evolve, leading to new discoveries and innovations in various scientific fields.

在微生物学领域，无菌培养这一概念指的是一种将生物体与其他物种隔离生长的方法。这一技术对于研究者来说至关重要，因为他们希望在没有其他干扰的情况下研究单一类型生物体的特征和行为。无菌培养是通过确保生长环境不受污染来实现的，这会显著影响实验结果。这一方法的重要性不言而喻，因为它使科学家能够获得纯净样本，这对准确分析至关重要。建立无菌培养的过程涉及几个步骤。首先，研究人员必须选择他们希望研究的生物体。这可以是细菌、真菌甚至植物细胞。一旦选择了生物体，下一步就是准备生长培养基。该培养基必须经过仔细灭菌，以消除可能污染培养的任何现有微生物。常用的灭菌技术包括高压蒸汽灭菌或使用化学消毒剂。在准备好培养基后，下一步是用所选生物体接种。此过程在无菌条件下进行，通常在层流罩内，以防止任何空气传播的污染物进入培养中。一旦生物体被引入培养基，它便开始生长和繁殖。在整个生长期间，密切监测培养物至关重要，以确保不存在任何不需要的生物体。任何污染迹象都必须立即处理，因为它们可能会危及无菌培养的完整性。使用无菌培养的主要优点之一是能够进行控制实验。例如，研究人员可以操纵温度、pH值和营养物质的可用性等环境因素，以观察这些变化如何影响生物体的生长和行为。这种控制水平在药理学等领域尤为重要，因为理解药物与特定微生物的相互作用对于开发有效治疗至关重要。此外，无菌培养在生物技术和农业中也发挥着重要作用。在生物技术中，它们用于生产酶、抗生素和其他生物活性化合物。通过孤立培养微生物，科学家可以最大化产量和纯度，这对于工业应用至关重要。在农业中，无菌培养用于研究植物病原体并开发抗病品种，从而提高作物产量和粮食安全。然而，尽管无菌培养有许多好处，但这种技术也面临挑战。保持整个过程的无菌状态可能很困难，即使是微小的技术失误也可能导致污染。此外，一些生物体由于其与其他物种的复杂关系而固有地难以孤立培养。在这种情况下，研究人员可能需要探索替代方法，例如共培养系统，以更好地理解这些相互作用。总之，创建和维持无菌培养的实践是微生物研究的基石。它为科学家提供了在受控环境中研究生物体的能力，免受外部影响。尽管存在需要克服的挑战，但无菌培养的好处远远超过缺点。随着研究的不断进展，围绕无菌培养的技术无疑会不断演变，从而在各个科学领域带来新的发现和创新。