alcohol precipitation

简明释义

酒精沉淀法

英英释义

例句

1.Researchers often rely on alcohol precipitation 醇沉淀 to concentrate nucleic acids from a solution.

研究人员常常依赖醇沉淀来从溶液中浓缩核酸。

2.After adding ethanol, we observed alcohol precipitation 醇沉淀 in the sample.

在样品中加入乙醇后，我们观察到了醇沉淀。

3.During the experiment, we performed alcohol precipitation 醇沉淀 to isolate the DNA.

在实验过程中，我们进行了醇沉淀以分离DNA。

4.The process of alcohol precipitation 醇沉淀 is commonly used in protein purification.

在蛋白质纯化中，醇沉淀的过程是常用的。

5.The efficiency of alcohol precipitation 醇沉淀 can vary depending on the concentration of alcohol used.

使用的酒精浓度会影响醇沉淀的效率。

作文

Alcohol precipitation is a widely used technique in biochemistry and molecular biology for the purification of proteins and nucleic acids. This method involves the addition of alcohol, typically ethanol or isopropanol, to a solution containing the target biomolecules. The principle behind alcohol precipitation (醇沉淀) is based on the solubility properties of the molecules in the presence of alcohol. When alcohol is added, it reduces the solubility of proteins and nucleic acids, causing them to aggregate and precipitate out of the solution. This process is crucial in many laboratory protocols where the goal is to isolate and concentrate specific biomolecules from complex mixtures.The procedure usually begins with the preparation of the sample, which may include cell lysates, culture supernatants, or other biological fluids. After clarifying the sample by centrifugation to remove debris, the appropriate volume of alcohol is added. The ratio of alcohol to the sample is critical and often varies depending on the specific biomolecule being targeted. For instance, a common ratio is 2:1 (alcohol to sample), but adjustments may be necessary based on empirical results.After the addition of alcohol, the mixture is typically incubated at low temperatures, often at -20°C or -80°C, for a period ranging from 30 minutes to several hours. This incubation promotes the formation of precipitates, which can then be collected by centrifugation. The resulting pellet contains the precipitated biomolecules, while the supernatant can be discarded or further analyzed if needed.Following centrifugation, the pellet is washed with cold alcohol to remove any contaminants or residual salts. It is important to ensure that the washing step is thorough, as impurities can affect downstream applications such as enzyme activity assays or sequencing. Once washed, the pellet is dried briefly to evaporate any remaining alcohol, and then it can be resuspended in an appropriate buffer for further use.One of the advantages of alcohol precipitation (醇沉淀) is its simplicity and cost-effectiveness. It does not require expensive reagents or sophisticated equipment, making it accessible to most laboratories. Additionally, this method is versatile and can be applied to various types of biomolecules, including proteins, DNA, and RNA. However, it is worth noting that not all proteins and nucleic acids precipitate equally well with alcohol, and some may require optimization of conditions for effective recovery.In conclusion, alcohol precipitation (醇沉淀) is an essential technique in the field of molecular biology, providing a straightforward means to purify biomolecules from complex mixtures. Its ease of use and adaptability make it a staple in many research and diagnostic laboratories. Understanding the principles and proper application of this technique can significantly enhance the efficiency and effectiveness of biomolecular studies.