agarose gel electrophoresis

简明释义

琼脂糖凝胶电泳

英英释义

例句

1.Using agarose gel electrophoresis, we were able to confirm the size of the amplified DNA.

通过使用琼脂糖凝胶电泳，我们能够确认放大DNA的大小。

2.The results of the agarose gel electrophoresis showed distinct bands for each sample.

该琼脂糖凝胶电泳的结果显示每个样本都有明显的条带。

3.The lab technician prepared the agarose gel electrophoresis setup before starting the experiment.

实验室技术员在开始实验之前准备了琼脂糖凝胶电泳的设备。

4.After performing agarose gel electrophoresis, we could visualize the PCR products under UV light.

在进行琼脂糖凝胶电泳后，我们可以在紫外光下观察PCR产物。

5.We used agarose gel electrophoresis to separate the DNA fragments for analysis.

我们使用琼脂糖凝胶电泳来分离DNA片段以进行分析。

作文

Agarose gel electrophoresis is a widely used technique in molecular biology for the separation of nucleic acids, such as DNA and RNA. This method relies on the movement of charged molecules through a gel matrix when an electric current is applied. The gel is made from agarose, a polysaccharide derived from seaweed, which forms a porous structure that allows smaller molecules to move more easily than larger ones. This property is crucial for the effective separation of nucleic acids based on their size. To perform agarose gel electrophoresis (琼脂糖凝胶电泳), scientists first prepare an agarose gel by dissolving agarose powder in a buffer solution and heating it until it becomes liquid. Once cooled, the gel is poured into a mold where it solidifies. After the gel has set, wells are created at one end to hold the samples. These samples typically contain DNA fragments that have been previously amplified using techniques like polymerase chain reaction (PCR). Once the samples are loaded into the wells, an electric current is applied across the gel. Because DNA is negatively charged due to its phosphate backbone, it will migrate towards the positive electrode. As the DNA moves through the gel, smaller fragments travel faster and farther than larger ones, leading to separation based on size. After a predetermined amount of time, the current is turned off, and the gel is stained with a DNA-binding dye, such as ethidium bromide or SYBR Green, which allows visualization of the DNA bands under ultraviolet light. The results of agarose gel electrophoresis (琼脂糖凝胶电泳) can be analyzed by comparing the position of the bands to a DNA ladder, which contains fragments of known sizes. This comparison helps researchers determine the sizes of the DNA fragments in their samples. This technique is essential in various applications, including genetic fingerprinting, cloning, and checking the quality of PCR products. In addition to its use in DNA analysis, agarose gel electrophoresis (琼脂糖凝胶电泳) can also be employed in RNA studies and protein separation, although other types of gels may be more suitable for proteins. Overall, this method is a cornerstone of molecular biology laboratories and has played a significant role in many scientific discoveries. In conclusion, agarose gel electrophoresis (琼脂糖凝胶电泳) is a fundamental technique that enables researchers to separate and analyze nucleic acids effectively. Its simplicity, efficiency, and versatility make it an indispensable tool in the field of molecular biology. Understanding how to perform and interpret the results of this technique is crucial for anyone working in genetic research or related areas. As technology advances, new modifications and enhancements to this technique continue to emerge, further expanding its applications and improving its resolution and sensitivity.