tetrazolium

简明释义

英[ˌtɛtrəˈzoʊliəm]美[ˌtɛtrəˈzoʊliəm]

n. 四唑

英英释义

单词用法

同义词

反义词

例句

1.GPA can also be used as a standard reference material to react with Nitro Blue Tetrazolium Chloride (NBT) in order to measure the glycosylated serum protein.

并以GPA作为标准参照物与氯化硝基四氯唑蓝(NBT)反应，用以测定糖化血清蛋白。

2.This homogeneous colorimetric assay is based on the conversion of a tetrazolium salt MTT, a pale yellow substrate, to formazan, a purple dye.

该匀相比色检测法是基于一种淡黄色底物四唑盐MTT可转化生成甲臜，甲臜是一种紫色显色剂。

3.NBT? corresponds to the melon extract quantity inhibiting 50% of NitroBlue Tetrazolium reduction. NBT?

对应于瓜提取量硝基四氮唑抑制减少50％。

4.Their inhibiting actions on the superoxide anions were examined by using nitroblue tetrazolium(NBT) method.

用NBT法研究了配合物对超氧阴离子自由基的抑制作用；

5.Using the tetrazolium-linked cytochemical method for estimation of glucose-6-phosphate dehydrogenase activity, the result indicate that extracted intercellular washing flu.

利用改造的对6-磷酸葡萄糖脱氢酶的硝基四氮唑蓝定性法对提取的细胞间隙液进行鉴定，结果显示得到了比较纯的细胞间隙液。

6.Cell viability was examined by methyl thiazolyl tetrazolium (MTT) assay and 50% inhibitive concentration (IC50) of cisplatin was examined also.

四甲基偶氮唑蓝比色法检测细胞存活率并计算顺铂的50%抑制浓度(IC50)。

7.Objective To introduce a quantitative colorimetric assay based on a tetrazolium salt (MTT).

目的介绍一种用四氮唑蓝（MTT）为底物的酶反应定量比色分析法。

8.The infarct size was measured by the triphenyl tetrazolium chloride method.

心肌梗塞面积通过三苯基四唑氯化物染色法测定。

9.Methyl thiazolyl tetrazolium (MTT) method was used to compare the ability of growth and proliferation between rat dental pulp stem cells and marrow mesenchymal stem cells.

采用四甲基偶氮唑盐法对大鼠牙髓干细胞和骨髓间充质干细胞的生长及增殖能力进行检测比较。

10.After treatment, the cells were incubated with tetrazolium 四唑盐 to measure metabolic activity.

处理后，细胞与四唑盐共同孵育以测量代谢活性。

11.The formation of a colored compound indicates the presence of tetrazolium 四唑盐 in the sample.

有色化合物的形成表明样品中存在四唑盐。

12.The researchers used tetrazolium 四唑盐 to assess cell viability in their experiments.

研究人员使用四唑盐来评估他们实验中的细胞活力。

13.In the colorimetric assay, tetrazolium 四唑盐 was reduced to a formazan product.

在比色法测定中，四唑盐被还原为福尔马赞产物。

14.The tetrazolium 四唑盐 test is commonly used in cancer research to evaluate drug efficacy.

在癌症研究中，四唑盐测试常用于评估药物的有效性。

作文

In the world of biochemistry and cellular biology, the term tetrazolium refers to a class of compounds that are often used as indicators in various biological assays. These compounds, particularly tetrazolium salts, have gained significant attention due to their ability to serve as electron acceptors in redox reactions. When reduced by viable cells, they form colored formazan products, which can be quantitatively measured. This property makes tetrazolium compounds particularly valuable in assessing cell viability, proliferation, and metabolic activity in different types of experiments.The most commonly used tetrazolium salt is 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT). MTT is widely employed in laboratory settings to determine the viability of cells in culture. When living cells are exposed to MTT, they reduce it to an insoluble purple formazan product. This reaction occurs because live cells possess active metabolic processes that facilitate the reduction of the tetrazolium compound. After a certain incubation period, the formazan crystals can be dissolved in a solvent, and the resulting solution's color intensity can be measured using a spectrophotometer. The higher the absorbance, the more viable cells are present in the sample.Another important aspect of tetrazolium compounds is their role in drug testing and cancer research. Researchers utilize the MTT assay to evaluate the cytotoxic effects of new pharmaceuticals on cancer cell lines. By determining how much a drug can reduce the viability of these cells, scientists can assess the potential effectiveness of the treatment. Furthermore, tetrazolium assays can help identify the mechanisms of action of various drugs, providing insights into how they affect cellular metabolism and growth.Beyond MTT, other tetrazolium compounds, such as XTT and WST-1, have been developed to improve upon the traditional MTT assay. These newer compounds offer advantages such as a more straightforward protocol and the ability to measure absorbance directly in the culture medium without the need for solubilization steps. Such advancements enhance the efficiency of experiments and allow for high-throughput screening of numerous samples simultaneously.In addition to their applications in cell viability assays, tetrazolium compounds have also been explored in the context of environmental science. For example, researchers have investigated the use of tetrazolium salts to assess the metabolic activity of microorganisms in soil or water samples. This approach can provide valuable information about the health of ecosystems and the impact of pollutants on microbial communities.In conclusion, tetrazolium compounds play a crucial role in various fields of biological research. Their ability to indicate cell viability and metabolic activity has made them indispensable tools in laboratories worldwide. As research continues to evolve, the applications of tetrazolium compounds are likely to expand further, leading to new discoveries and advancements in science. Understanding the significance of tetrazolium in research not only highlights its importance in assessing cell health but also underscores the broader implications for drug development and environmental monitoring.

在生物化学和细胞生物学的世界中，术语tetrazolium指的是一类化合物，通常用作各种生物检测中的指示剂。这些化合物，特别是tetrazolium盐，由于其作为氧化还原反应中电子受体的能力而受到广泛关注。当活细胞还原这些化合物时，它们会形成有色的福尔马赞产物，这些产物可以定量测量。这种特性使得tetrazolium化合物在评估细胞活力、增殖和代谢活动方面非常有价值。最常用的tetrazolium盐是3-(4,5-二甲基噻唑-2-基)-2,5-二苯基tetrazolium溴化物（MTT）。MTT在实验室中广泛使用，以确定培养中细胞的活力。当活细胞暴露于MTT时，它们将其还原为不溶性紫色福尔马赞产物。这个反应发生是因为活细胞具有活跃的代谢过程，可以促进tetrazolium化合物的还原。在一定的孵育时间后，福尔马赞晶体可以溶解在溶剂中，得到的溶液的颜色强度可以使用分光光度计进行测量。吸光度越高，样本中存在的活细胞就越多。tetrazolium化合物的另一个重要方面是它们在药物测试和癌症研究中的作用。研究人员利用MTT实验评估新药对癌细胞系的细胞毒性影响。通过确定药物能够减少这些细胞活力的程度，科学家可以评估治疗的潜在有效性。此外，tetrazolium实验可以帮助识别各种药物的作用机制，提供关于它们如何影响细胞代谢和生长的见解。除了MTT之外，其他tetrazolium化合物，如XTT和WST-1，已被开发出来，以改进传统的MTT实验。这些新化合物提供了诸如更简单的实验步骤以及能够直接在培养基中测量吸光度而无需溶解步骤等优点。这些进展提高了实验的效率，并允许同时对大量样本进行高通量筛选。除了在细胞活力实验中的应用外，tetrazolium化合物还在环境科学中得到了探索。例如，研究人员调查了使用tetrazolium盐来评估土壤或水样中微生物的代谢活性。这种方法可以提供有关生态系统健康和污染物对微生物群落影响的宝贵信息。总之，tetrazolium化合物在各个生物研究领域中发挥着至关重要的作用。它们指示细胞活力和代谢活动的能力使它们成为全球实验室中不可或缺的工具。随着研究的不断发展，tetrazolium化合物的应用可能会进一步扩展，带来新的发现和科学进步。理解tetrazolium在研究中的重要性不仅突显了它在评估细胞健康方面的重要性，还强调了其对药物开发和环境监测的更广泛影响。