hybridoma

简明释义

英[ˌhaɪbrəˈdəʊmə]美[ˌhaɪbrɪˈdoʊmə]

n. 杂种瘤；杂种细胞（细胞融合后形成的）

复 数 h y b r i d o m a s

英英释义

单词用法

同义词

反义词

例句

1.At last, we got one positive hybridoma cells named 4d11.

筛选共得到一株阳性杂交瘤细胞，命名为4d11。

2.Methods The titer and affinity of anti-A secreted by hybridoma were tested by tube method and slide method.

通过试管法和玻片法检测抗a抗体的效价和亲和力。

3.The splenocytes of immunized BALB/c strain mice with purified Ig were fused with myeloma cell line, NS-1 . The supernatants of hybridoma were screened by ELISA.

将纯化之球蛋白片段免疫 BALB/c小鼠后， 取脾脏细胞与小鼠骨髓瘤细胞株 NS-1进行细胞融合。

4.Electrical parameters affecting gene transfer, such as the field strength ( Voltage ), pulse durations and pulse frequency ect. and optimum transfer conditions in murine hybridoma cells were studied.

研究了电场强度、脉冲宽度、脉冲次数等电场参数对基因转化频率的影响和小鼠杂交瘤细胞最适电转移条件。

5.The principles of hybridoma technique are involved in some advantages, high specificity, sensitivity, convenience and quickness.

杂交瘤技术原理和操作过程包含了其许多优点，特异性强、方便、敏感和快速。

6.Objective: To obtain hybridoma cell line which can secrete anti-gonyautoxin2,3 (GTX2,3) monoclonal antibodies(mAb) and analysis the character of the mAb.

目的：建立抗麻痹性贝毒素GTX2，3单克隆抗体杂交瘤细胞系，制备抗GTX2，3单克隆抗体，并鉴定其免疫学特性。

7.We have obtained the second group of monoclonal antibodies against human platelet by means of hybridoma technique.

用杂交瘤技术获得了第二组抗人血小板的单克隆抗体。

8.A successful fusion of myeloma cells and spleen cells resulted in a viable hybridoma 杂交瘤细胞 line.

骨髓瘤细胞和脾细胞的成功融合产生了一个可行的hybridoma 杂交瘤细胞系。

9.The study focused on the characterization of the newly created hybridoma 杂交瘤细胞 for therapeutic use.

该研究集中在新创建的hybridoma 杂交瘤细胞的特征化，以便于治疗使用。

10.The hybridoma 杂交瘤细胞 technology has revolutionized the field of immunology.

hybridoma 杂交瘤细胞技术彻底改变了免疫学领域。

11.The scientist developed a new hybridoma 杂交瘤细胞 to produce a specific antibody.

科学家开发了一种新的hybridoma 杂交瘤细胞以生产特定的抗体。

12.Using hybridomas 杂交瘤细胞, researchers can create monoclonal antibodies for medical diagnostics.

研究人员可以使用hybridomas 杂交瘤细胞来创建用于医学诊断的单克隆抗体。

作文

The field of biotechnology has seen significant advancements over the past few decades, one of which is the development of monoclonal antibodies. These are antibodies that are identical and produced by a single clone of cells. The process of creating these antibodies involves a technique known as hybridoma technology. A hybridoma is a type of cell that is created by fusing a specific type of immune cell, called a B cell, with a myeloma (cancer) cell. This fusion results in a cell that can both produce a specific antibody and replicate indefinitely, making it a powerful tool in research and medicine.The significance of hybridoma technology cannot be overstated. It allows scientists to produce large quantities of a specific antibody, which can then be used for various applications, including diagnostic tests, therapeutic treatments, and research purposes. For instance, in the medical field, monoclonal antibodies derived from hybridomas have been used to treat diseases such as cancer, autoimmune disorders, and infectious diseases.The process of creating a hybridoma begins with the immunization of a mouse or another suitable animal with an antigen. This antigen triggers the animal's immune system to produce B cells that generate antibodies against the antigen. After sufficient immune response is achieved, the spleen cells containing these B cells are harvested. These B cells are then fused with myeloma cells using a special chemical or electrical method. The resulting hybrid cells, or hybridomas, are capable of producing the desired antibody while also having the ability to grow indefinitely in culture.Once the hybridomas are formed, they are screened for the production of the specific antibody of interest. This screening process is crucial, as not all hybrid cells will produce the desired antibody. Once identified, the successful hybridoma cells can be cloned, allowing for the production of large quantities of the antibody. This process has revolutionized the way antibodies are produced and has made it possible to develop targeted therapies that were previously unimaginable.Moreover, the applications of hybridoma technology extend beyond medicine. In research laboratories, monoclonal antibodies are used to study proteins and other molecules, helping scientists understand biological processes at a deeper level. They are also utilized in various industries, from agriculture to environmental science, where they can help detect pathogens or toxins.Despite its numerous advantages, there are challenges associated with hybridoma technology. For example, producing monoclonal antibodies can be expensive and time-consuming. Additionally, there can be issues related to the specificity and affinity of the antibodies produced, which may require further optimization. Nonetheless, ongoing research aims to address these challenges and improve the efficiency and effectiveness of hybridoma technology.In conclusion, the invention of hybridoma technology has had a profound impact on science and medicine. It has enabled the production of monoclonal antibodies that play a crucial role in diagnostics and therapeutics. As research continues to advance, we can expect to see even more innovative applications of hybridoma technology, leading to improved health outcomes and a better understanding of complex biological systems.

生物技术领域在过去几十年中取得了显著进展，其中之一就是单克隆抗体的开发。这些抗体是相同的，并由单一细胞克隆产生。创建这些抗体的过程涉及一种称为杂交瘤技术的技术。杂交瘤是一种通过将特定类型的免疫细胞（称为B细胞）与骨髓瘤（癌细胞）融合而形成的细胞。这种融合产生了一种既能产生特定抗体又能无限复制的细胞，使其成为研究和医学中的强大工具。杂交瘤技术的重要性不容小觑。它使科学家能够大量生产特定抗体，这些抗体可以用于各种应用，包括诊断测试、治疗和研究目的。例如，在医学领域，来自杂交瘤的单克隆抗体已被用于治疗癌症、自身免疫疾病和传染病等疾病。创建杂交瘤的过程始于对小鼠或其他合适动物进行免疫接种，使用抗原。该抗原刺激动物的免疫系统产生生成针对抗原抗体的B细胞。在达到足够的免疫反应后，收获含有这些B细胞的脾脏细胞。然后，使用特殊的化学物质或电气方法将这些B细胞与骨髓瘤细胞融合。最终形成的杂交细胞，即杂交瘤，既能产生所需抗体，又能在培养中无限生长。一旦形成了杂交瘤，就会对其进行筛选，以寻找产生特定抗体的细胞。这个筛选过程至关重要，因为并非所有的杂交细胞都会产生所需的抗体。一旦确定，成功的杂交瘤细胞可以被克隆，从而允许生产大量抗体。这个过程彻底改变了抗体的生产方式，使得开发以前无法想象的靶向疗法成为可能。此外，杂交瘤技术的应用不仅限于医学。在研究实验室中，单克隆抗体用于研究蛋白质和其他分子，帮助科学家更深入地理解生物过程。它们还被广泛应用于农业和环境科学等各个行业，有助于检测病原体或毒素。尽管有许多优点，但杂交瘤技术也面临挑战。例如，生产单克隆抗体可能成本高昂且耗时。此外，生产的抗体的特异性和亲和力可能存在问题，这可能需要进一步优化。然而，持续的研究旨在解决这些挑战，提高杂交瘤技术的效率和有效性。总之，杂交瘤技术的发明对科学和医学产生了深远的影响。它使得单克隆抗体的生产成为可能，这些抗体在诊断和治疗中发挥着至关重要的作用。随着研究的不断进展，我们可以期待看到杂交瘤技术的更多创新应用，从而改善健康结果，更好地理解复杂的生物系统。