phase contrast

简明释义

相差

英英释义

例句

1.The scientist used phase contrast microscopy to observe live cells without staining them.

科学家使用相位对比显微镜观察活细胞而无需染色。

2.The phase contrast method is essential for examining transparent biological samples.

对于检查透明生物样本，相位对比方法是必不可少的。

3.Using phase contrast imaging, researchers can detect subtle differences in cell structures.

通过使用相位对比成像，研究人员可以检测到细胞结构的微妙差异。

4.The phase contrast technique allows us to visualize living tissues in their natural state.

该相位对比技术使我们能够在自然状态下可视化活组织。

5.In the laboratory, phase contrast techniques help in studying the morphology of microorganisms.

在实验室中，相位对比技术有助于研究微生物的形态。

作文

The world of microscopy has evolved significantly over the years, leading to advancements that allow scientists to observe the intricate details of living cells. One such advancement is the technique known as phase contrast, which enhances the visibility of transparent specimens without the need for staining. This method utilizes differences in the refractive index of cellular components to produce contrast in the image. The significance of phase contrast cannot be overstated, as it has revolutionized the way biologists study live cells and their dynamics.When light passes through a specimen, different parts of the cell can bend the light waves differently due to variations in density and composition. Traditional microscopy often struggles to visualize these subtle differences, as unstained cells appear nearly transparent. However, with phase contrast microscopy, these differences are converted into visible contrasts that make it easier to see structures like nuclei, organelles, and even the movement of cellular processes in real-time.This technique was first developed by Dutch physicist Frits Zernike in the 1930s, who later received the Nobel Prize in Physics for his work. His invention allowed scientists to observe live cells in their natural state, leading to groundbreaking discoveries in cell biology and microbiology. For instance, researchers can now monitor the behavior of bacteria, yeast, and other microorganisms as they grow and divide, providing insights into their life cycles and interactions.One of the most remarkable applications of phase contrast microscopy is in the field of medical research. It enables scientists to study cancer cells and their responses to various treatments without altering their natural state. By observing how these cells behave under different conditions, researchers can identify potential therapeutic targets and develop new treatment strategies. Moreover, phase contrast has also been instrumental in developmental biology, allowing scientists to track the growth and differentiation of stem cells.In addition to its applications in research, phase contrast microscopy is also widely used in clinical settings. Pathologists utilize this technique to examine tissue samples, helping to diagnose diseases and guide treatment decisions. The ability to visualize cellular structures clearly aids in identifying abnormalities that may indicate the presence of disease.Despite its many advantages, phase contrast microscopy does have limitations. The technique can sometimes produce halo effects around the edges of cells, which may obscure certain details. Additionally, not all specimens are suitable for phase contrast imaging, particularly those that are highly pigmented or opaque. Nevertheless, ongoing advancements in microscopy technology continue to improve the capabilities of phase contrast techniques, making them even more valuable in scientific research and clinical practice.In conclusion, the development of phase contrast microscopy has significantly enhanced our ability to observe and understand the complexities of living cells. Its applications in research and medicine underscore its importance in advancing our knowledge of biology and improving health outcomes. As technology progresses, we can expect phase contrast microscopy to play an even more critical role in scientific exploration and discovery, opening new avenues for understanding life at the cellular level.

显微镜学的世界随着时间的推移发生了显著的发展，使科学家能够观察到活细胞的复杂细节。其中一种进步是被称为相位对比的技术，它在不需要染色的情况下增强透明标本的可见性。这种方法利用细胞组分的折射率差异来产生图像中的对比度。相位对比的重要性不容小觑，因为它彻底改变了生物学家研究活细胞及其动态的方式。当光线穿过标本时，细胞的不同部分由于密度和成分的变化可以以不同的方式弯曲光波。传统显微镜通常难以可视化这些微妙的差异，因为未染色的细胞几乎是透明的。然而，通过相位对比显微镜，这些差异被转换为可见的对比，使得观察细胞核、细胞器甚至细胞过程的运动变得更加容易。这种技术最早由荷兰物理学家弗里茨·泽尔尼克于20世纪30年代开发，他因这项工作获得了诺贝尔物理学奖。他的发明使科学家能够观察到活细胞的自然状态，从而导致细胞生物学和微生物学领域的突破性发现。例如，研究人员现在可以监测细菌、酵母和其他微生物在生长和分裂过程中的行为，从而提供对其生命周期和相互作用的见解。相位对比显微镜的一个显著应用是在医学研究领域。它使科学家能够研究癌细胞及其对各种治疗的反应，而不改变其自然状态。通过观察这些细胞在不同条件下的行为，研究人员可以识别潜在的治疗靶点并开发新的治疗策略。此外，相位对比在发育生物学中也发挥了重要作用，使科学家能够追踪干细胞的生长和分化。除了在研究中的应用外，相位对比显微镜在临床环境中也被广泛使用。病理学家利用这一技术检查组织样本，帮助诊断疾病并指导治疗决策。清晰可视化细胞结构的能力有助于识别可能表明疾病存在的异常。尽管有许多优点，相位对比显微镜也有局限性。这种技术有时会在细胞边缘产生光晕效应，这可能会遮蔽某些细节。此外，并非所有标本都适合相位对比成像，特别是那些高度着色或不透明的标本。尽管如此，显微镜技术的持续进步仍在不断改善相位对比技术的能力，使其在科学研究和临床实践中更具价值。总之，相位对比显微镜的发展显著增强了我们观察和理解活细胞复杂性的能力。它在研究和医学中的应用强调了它在推动我们生物学知识和改善健康结果方面的重要性。随着技术的进步，我们可以期待相位对比显微镜在科学探索和发现中发挥更关键的作用，为理解细胞层面的生命开辟新的途径。