cycloheximide

简明释义

英[ˌsaɪkləʊˈheksəˌmaɪd]美[ˌsaɪkloʊˈheksəˌmaɪd]

n. [药] 环己酰亚胺；[农药] 放线菌酮

英英释义

单词用法

同义词

反义词

例句

1.Having treated roots with PEG and Actinomycin D or Cycloheximide, the accumulation of ABA in roots and leaves were inhibited clearly.

经放线菌素D和环己亚胺分别与PEG一起处理，根及叶片ABA增加均明显受抑制，说明此ABA增加可能来源于组织的从头生物合。

2.Method:The learning and memory tests were realized with a water maze on the learning and memory impact of mice induced by scopolamine and cycloheximide.

方法：采用东莨菪碱及环己亚酰胺致小鼠学习记忆障碍模型，以水迷宫为学习记忆评价指标。并观察该药对小鼠自主活动性和抗疲劳能力的影响。

3.Cycloheximide(CHX), a protein synthesis inhibitor, can activate mouse eggs but the underlying mechanism is not fully known.

蛋白合成抑制剂亚胺环己酮可以诱导小鼠卵母细胞发生孤雌活化，但其机制尚未完全阐明。

4.Cycloheximide inhibited both the increase of soluble protein and the development of freezing tolerance.

环已亚胺抑制可溶性蛋白质含量增加，同时也抑制细胞抗冻性发育。

5.Using the rats treated with cycloheximide as experimental models of apoptosis, lymphocyte ultrastructures of the thymus, spleen and lymph nodes were observed with transmission electron microscope.

本实验用放线菌酮处理大鼠建造细胞调亡模型。用透射电镜观察大鼠胸腺、脾脏和肠系膜淋巴结淋巴细胞的超微结构。

6.Using the rats treated with cycloheximide as experimental models of apoptosis, lymphocyte ultrastructures of the thymus, spleen and lymph nodes were observed with transmission electron microscope.

本实验用放线菌酮处理大鼠建造细胞调亡模型。用透射电镜观察大鼠胸腺、脾脏和肠系膜淋巴结淋巴细胞的超微结构。

7.The effect of cycloheximide on cell viability was assessed in the study.

在研究中评估了环己酰亚胺对细胞活力的影响。

8.After treatment with cycloheximide, the cells showed decreased protein levels.

经过环己酰亚胺处理后，细胞显示出蛋白质水平下降。

9.Inhibiting translation with cycloheximide can help understand gene function.

使用环己酰亚胺抑制翻译有助于理解基因功能。

10.The team added cycloheximide to the culture medium to study its effects.

团队向培养基中添加了环己酰亚胺以研究其效果。

11.Researchers used cycloheximide to inhibit protein synthesis in their experiments.

研究人员使用环己酰亚胺抑制他们实验中的蛋白质合成。

作文

Cycloheximide is a potent inhibitor of protein synthesis in eukaryotic cells, and it has been extensively used in biological research. This compound works by interfering with the translocation step of protein synthesis, effectively halting the process. As scientists delve deeper into the mechanisms of cellular function, understanding the role of inhibitors like cycloheximide becomes crucial. In particular, cycloheximide is frequently employed in experiments to study gene expression and protein turnover. By inhibiting protein synthesis, researchers can observe the effects on cellular processes and better understand how proteins contribute to various functions within the cell.One of the significant applications of cycloheximide is in the study of apoptosis, or programmed cell death. When cells undergo apoptosis, specific proteins are synthesized or degraded, and using cycloheximide allows researchers to determine the timing and necessity of these proteins in the apoptotic pathway. For example, if the addition of cycloheximide prevents cell death, it may indicate that the synthesis of certain pro-apoptotic factors is essential for this process. Conversely, if cells continue to die in the presence of cycloheximide, it suggests that the apoptotic machinery is already activated and does not rely on new protein synthesis.Moreover, cycloheximide can be instrumental in studying the effects of various drugs on cancer cells. Many anticancer therapies target rapidly dividing cells, and understanding how these treatments interact with protein synthesis pathways can provide insights into their efficacy. By using cycloheximide, researchers can analyze whether the inhibition of protein synthesis affects the response of cancer cells to these drugs, potentially leading to improved treatment strategies.In addition to its applications in apoptosis and cancer research, cycloheximide is also utilized in studies related to cellular stress responses. Cells often encounter stressful conditions, such as oxidative stress or nutrient deprivation, which can lead to changes in protein synthesis. By applying cycloheximide, scientists can investigate how cells adapt to these stressors and identify key proteins involved in the stress response. This knowledge can contribute to our understanding of diseases where stress responses are dysregulated, such as neurodegenerative disorders.Despite its widespread use, researchers must exercise caution when using cycloheximide in experiments. The concentration of cycloheximide must be carefully controlled, as excessive amounts can lead to non-specific effects that complicate the interpretation of results. Additionally, since cycloheximide is toxic to cells, its application should be limited to specific time frames to minimize cell death unrelated to the experimental question.In conclusion, cycloheximide serves as a valuable tool in the field of molecular biology, particularly in studies related to protein synthesis, apoptosis, cancer research, and cellular stress responses. Its ability to inhibit protein synthesis allows researchers to dissect complex cellular processes and gain insights into the roles of specific proteins. As research continues to advance, the applications of cycloheximide will likely expand, providing further understanding of cellular mechanisms and potential therapeutic targets for various diseases.

环己酰亚胺是一种强效的真核细胞蛋白合成抑制剂，广泛应用于生物研究中。该化合物通过干扰蛋白合成的转位步骤来发挥作用，有效地停止这一过程。随着科学家们对细胞功能机制的深入研究，理解像环己酰亚胺这样的抑制剂的作用变得至关重要。特别是，环己酰亚胺常用于研究基因表达和蛋白质周转的实验中。通过抑制蛋白合成，研究人员可以观察对细胞过程的影响，更好地理解蛋白质在细胞内各种功能中的作用。环己酰亚胺的一个重要应用是在凋亡或程序性细胞死亡的研究中。当细胞经历凋亡时，特定蛋白的合成或降解会发生，使用环己酰亚胺可以让研究人员确定这些蛋白在凋亡途径中的时机和必要性。例如，如果添加环己酰亚胺阻止细胞死亡，这可能表明某些促凋亡因子的合成对于这一过程至关重要。相反，如果细胞在环己酰亚胺存在的情况下仍然继续死亡，则表明凋亡机制已经被激活，并不依赖于新的蛋白质合成。此外，环己酰亚胺在研究各种药物对癌细胞影响方面也具有重要意义。许多抗癌治疗针对快速分裂的细胞，理解这些治疗与蛋白合成途径的相互作用可以为其有效性提供见解。通过使用环己酰亚胺，研究人员可以分析抑制蛋白合成是否影响癌细胞对这些药物的反应，从而可能导致改进的治疗策略。除了在凋亡和癌症研究中的应用，环己酰亚胺还用于与细胞应激反应相关的研究。细胞经常遇到压力条件，例如氧化压力或营养缺乏，这可能导致蛋白合成的变化。通过施加环己酰亚胺，科学家可以研究细胞如何适应这些压力源，并识别参与应激反应的关键蛋白。这些知识可以帮助我们理解应激反应失调的疾病，例如神经退行性疾病。尽管环己酰亚胺被广泛使用，但研究人员在实验中使用时必须谨慎。环己酰亚胺的浓度必须严格控制，因为过量可能导致非特异性效应，从而使结果的解释复杂化。此外，由于环己酰亚胺对细胞有毒，因此其应用应限制在特定时间范围内，以最小化与实验问题无关的细胞死亡。总之，环己酰亚胺在分子生物学领域中是一种宝贵的工具，特别是在与蛋白合成、凋亡、癌症研究和细胞应激反应相关的研究中。它抑制蛋白合成的能力使研究人员能够剖析复杂的细胞过程，并获得对特定蛋白作用的见解。随着研究的不断推进，环己酰亚胺的应用可能会扩展，从而进一步理解细胞机制和各种疾病的潜在治疗靶点。