fluorometric analysis

简明释义

荧光测定

英英释义

例句

1.In environmental studies, researchers often use fluorometric analysis 荧光分析 to detect pollutants in water samples.

在环境研究中，研究人员经常使用fluorometric analysis 荧光分析来检测水样中的污染物。

2.The lab implemented fluorometric analysis 荧光分析 to improve the accuracy of their genetic testing.

实验室实施了fluorometric analysis 荧光分析以提高基因检测的准确性。

3.Using fluorometric analysis 荧光分析, scientists can measure the levels of specific proteins in cell cultures.

通过使用fluorometric analysis 荧光分析，科学家可以测量细胞培养中特定蛋白质的水平。

4.The pharmaceutical industry relies on fluorometric analysis 荧光分析 for quantifying drug concentrations in biological fluids.

制药行业依赖于fluorometric analysis 荧光分析来定量生物体液中的药物浓度。

5.In clinical diagnostics, fluorometric analysis 荧光分析 is used to identify biomarkers for various diseases.

在临床诊断中，fluorometric analysis 荧光分析用于识别各种疾病的生物标志物。

作文

Fluorometric analysis is a powerful analytical technique that relies on the measurement of fluorescence emitted by a sample after it has absorbed light. This method is widely used in various fields, including biochemistry, environmental science, and clinical diagnostics, due to its high sensitivity and specificity. The basic principle of fluorometric analysis (荧光分析) involves exciting a molecule with a light source, typically a UV or visible light, causing the molecule to emit light at a different wavelength. This emitted light is then measured and analyzed to determine the concentration of the target analyte in the sample.One of the primary advantages of fluorometric analysis (荧光分析) is its ability to detect very low concentrations of substances, often in the nanomolar range. This makes it an invaluable tool in research and clinical settings where precise measurements are crucial. For example, in medical diagnostics, fluorometric analysis (荧光分析) can be used to detect biomarkers associated with diseases, enabling early diagnosis and treatment.The process of fluorometric analysis (荧光分析) typically begins with the preparation of the sample, which may involve dilution or extraction steps to isolate the analyte of interest. Once prepared, the sample is placed in a fluorometer, an instrument designed to measure fluorescence. The fluorometer emits light of a specific wavelength, exciting the molecules in the sample. As these molecules return to their ground state, they emit light, which is collected and quantified by the instrument.In addition to its sensitivity, fluorometric analysis (荧光分析) is also praised for its rapid turnaround time. Many assays can be completed within a few hours, making it suitable for high-throughput screening applications. This speed is particularly beneficial in clinical labs where timely results can significantly impact patient care.Despite its many advantages, there are some limitations to fluorometric analysis (荧光分析). One challenge is the potential for interference from other substances present in the sample, which can affect the accuracy of the results. To mitigate this, researchers often employ controls and calibrations to ensure that the measurements reflect the true concentration of the analyte. Additionally, the choice of fluorophore, the fluorescent molecule used in the assay, is critical, as different fluorophores have varying excitation and emission properties that can influence the overall effectiveness of the analysis.Overall, fluorometric analysis (荧光分析) is an essential technique in modern science, providing researchers and clinicians with a reliable method for quantifying substances in complex mixtures. Its applications range from monitoring environmental pollutants to diagnosing diseases, showcasing its versatility and importance in both research and practical applications. As technology advances, we can expect further improvements in fluorometric analysis (荧光分析), leading to even more sensitive and efficient methods for analyzing a wide array of samples. The continued development of novel fluorophores and analytical techniques will undoubtedly enhance the capabilities of this already robust method, solidifying its role as a cornerstone of analytical chemistry.

荧光分析是一种强大的分析技术，依赖于测量样品在吸收光线后发出的荧光。这种方法因其高灵敏度和特异性而广泛应用于生物化学、环境科学和临床诊断等多个领域。荧光分析的基本原理涉及用光源（通常是紫外线或可见光）激发分子，使分子以不同波长发出光。然后测量和分析这些发出的光，以确定样品中目标分析物的浓度。荧光分析的主要优点之一是能够检测到非常低浓度的物质，通常在纳摩尔范围内。这使其成为研究和临床环境中不可或缺的工具，在这些环境中，精确的测量至关重要。例如，在医学诊断中，荧光分析可以用于检测与疾病相关的生物标志物，从而实现早期诊断和治疗。荧光分析的过程通常从样品的准备开始，这可能涉及稀释或提取步骤，以分离所关注的分析物。一旦准备好，样品就会放置在荧光仪中，该仪器旨在测量荧光。荧光仪发出特定波长的光，激发样品中的分子。当这些分子返回基态时，会发出光，该光被仪器收集并量化。除了灵敏度，荧光分析还因其快速的周转时间而受到赞誉。许多测定可以在几个小时内完成，使其适合高通量筛选应用。这种速度在临床实验室中特别有益，因为及时的结果可以显著影响患者护理。尽管有许多优点，荧光分析也存在一些局限性。其中一个挑战是样品中其他物质的潜在干扰，这可能影响结果的准确性。为了减轻这一问题，研究人员通常采用对照和校准，以确保测量反映分析物的真实浓度。此外，荧光探针的选择，即在测定中使用的荧光分子，是至关重要的，因为不同的荧光探针具有不同的激发和发射特性，这可能会影响分析的整体有效性。总体而言，荧光分析是现代科学中一种重要的技术，为研究人员和临床医生提供了一种可靠的方法来定量复杂混合物中的物质。它的应用范围从监测环境污染物到诊断疾病，展示了其在研究和实际应用中的多功能性和重要性。随着技术的发展，我们可以期待对荧光分析的进一步改进，从而为分析各种样品提供更灵敏和高效的方法。新型荧光探针和分析技术的持续发展无疑将增强这一已经强大的方法的能力，巩固其作为分析化学基石的角色。