transfect

简明释义

英[trænzˈfekt;trɑːnz-;-ns-]美[trænzˈfekt]

vt. 使转染；使细胞感染病毒核酸

英英释义

单词用法

同义词

反义词

例句

1.Results Artificial synthetic DNA transfer system and modified viral vectors could efficiently transfect target cells and get high level expression.

结果合成的DNA转运系统和经修饰的病毒载体体外转染靶细胞后，可获得显著表达。

2.Polymeric carrier containing folic acid is expected to be able to identify tumor surface receptors and transfect cells by receptor-mediated endocytosis.

含有叶酸的聚合载体能够识别肿瘤表面受体，并且能够通过受体介导的(细胞)内摄作用转染细胞。

3.It could be expected that nucleoprotamine modified calcium phosphate could be a better transfect agent as compared to coprecipitated superphosphate.

本研究认为鱼精蛋白改性磷酸钙是一种比共沉淀法合成磷酸钙更好的转染载体。

4.Objective To construct eukaryotic expressing vector of human melanin-concentrating hormone receptor 1(MCHR1), then to transfect CHO cells with the vector for establishment of stable CHO cell line.

目的构建人黑色素浓集激素1型受体（MCHR1）真核表达载体，转染CHO细胞，建立稳定转染的CHO细胞系。

5.The aim of gene therapy is to transfect DNA into target cells, in which utilizing antisense oligonucleotides is a main method.

基因治疗是将DNA转染进入目的细胞，修复遗传错误或产生治疗因子。反义寡核苷酸的应用是基因治疗的主要手段之一。

6.Objective To construct eukaryotic expressing vector of human melanin-concentrating hormone receptor 1(MCHR1), then to transfect CHO cells with the vector for establishment of stable CHO cell line.

目的构建人黑色素浓集激素1型受体（MCHR1）真核表达载体，转染CHO细胞，建立稳定转染的CHO细胞系。

7.To study protein function, scientists need to transfect 转染 the target cells with the corresponding DNA.

为了研究蛋白质功能，科学家需要将相应的DNA转染转染到目标细胞中。

8.Researchers often use plasmids to transfect 转染 mammalian cells for gene expression studies.

研究人员通常使用质粒来转染转染哺乳动物细胞以进行基因表达研究。

9.They utilized lipofection to transfect 转染 the neurons in their experiment.

他们在实验中利用脂质转染法来转染转染神经元。

10.The efficiency of transfection 转染 can vary depending on the cell type used.

不同细胞类型的转染转染效率可能会有所不同。

11.The team successfully transfected 转染 the cancer cells with a therapeutic gene.

团队成功地将治疗基因转染转染到癌细胞中。

作文

In the realm of molecular biology, the term transfect refers to the process of introducing nucleic acids into cells. This technique is essential for various applications, including gene expression studies, the development of vaccines, and gene therapy. The ability to transfect cells allows researchers to manipulate genetic material, enabling them to observe the effects of specific genes on cellular functions. For instance, scientists can introduce a plasmid containing a gene of interest into a cell line to study how that gene influences protein production or cellular behavior.The methods used to transfect cells have evolved significantly over the years. Early techniques relied on physical methods such as electroporation, where an electric field is applied to increase cell permeability, allowing DNA to enter the cell. Another common method is lipofection, which involves using lipid-based reagents that encapsulate the nucleic acids and facilitate their entry into cells. These advancements have made it easier to transfect a wide variety of cell types, including hard-to-transfect primary cells and stem cells.One of the most exciting applications of transfecting cells is in the field of gene therapy. By delivering therapeutic genes into patients' cells, researchers aim to correct genetic disorders at the molecular level. For example, in certain inherited diseases where a defective gene causes health issues, scientists can transfect healthy copies of the gene into the patient's cells to restore normal function. This approach holds great promise for treating conditions such as cystic fibrosis, muscular dystrophy, and some forms of cancer.Moreover, the ability to transfect cells has also paved the way for vaccine development. During the COVID-19 pandemic, mRNA vaccines were developed using a technique that involves transfecting human cells with messenger RNA that instructs the body to produce a harmless piece of the virus, triggering an immune response. This innovative approach demonstrated how effective transfection could be in rapidly addressing public health crises.Despite its advantages, transfection is not without challenges. One significant hurdle researchers face is achieving high efficiency while minimizing potential cytotoxicity. Cells can be sensitive to the reagents used during the transfection process, which may lead to cell death or altered cellular functions. Therefore, optimizing transfection protocols is crucial for ensuring that the desired genetic material is delivered effectively without harming the cells.In conclusion, the process of transfecting cells is a powerful tool in molecular biology that has transformed our understanding of genetics and paved the way for innovative therapies. From basic research to clinical applications, the ability to manipulate genetic material within cells has far-reaching implications for medicine and biotechnology. As techniques continue to improve, we can expect even more groundbreaking discoveries and advancements in the future, all thanks to the fundamental process of transfection.

在分子生物学领域，术语transfect指的是将核酸引入细胞的过程。这项技术对于各种应用至关重要，包括基因表达研究、疫苗开发和基因治疗。能够transfect细胞使研究人员能够操纵遗传材料，从而观察特定基因对细胞功能的影响。例如，科学家可以将含有感兴趣基因的质粒引入细胞系，以研究该基因如何影响蛋白质生产或细胞行为。用于transfect细胞的方法多年来发生了显著变化。早期技术依赖于物理方法，如电穿孔，在施加电场的情况下增加细胞通透性，使DNA能够进入细胞。另一种常见方法是脂质转染，它涉及使用脂质基试剂包裹核酸并促进其进入细胞。这些进展使得transfect各种细胞类型变得更加容易，包括难以转染的原代细胞和干细胞。Transfect细胞的最令人兴奋的应用之一是在基因治疗领域。通过将治疗基因送入患者细胞，研究人员旨在从分子层面纠正遗传疾病。例如，在某些遗传疾病中，缺陷基因导致健康问题，科学家们可以将健康的基因拷贝transfect到患者的细胞中，以恢复正常功能。这种方法在治疗囊性纤维化、肌营养不良症和某些形式的癌症等疾病方面具有巨大前景。此外，能够transfect细胞还为疫苗开发铺平了道路。在COVID-19大流行期间，mRNA疫苗的开发采用了一种技术，该技术涉及将信使RNAtransfect人类细胞，指示身体产生病毒的无害部分，从而触发免疫反应。这种创新方法展示了transfection在快速应对公共卫生危机中的有效性。尽管有其优势，transfection仍然面临挑战。研究人员面临的一个重大障碍是实现高效率，同时尽量减少潜在的细胞毒性。细胞对在transfection过程中使用的试剂可能很敏感，这可能导致细胞死亡或细胞功能改变。因此，优化transfection方案对于确保所需遗传材料有效传递而不损害细胞至关重要。总之，transfect细胞的过程是一种强大的分子生物学工具，改变了我们对遗传学的理解，并为创新疗法铺平了道路。从基础研究到临床应用，能够操纵细胞内的遗传材料对医学和生物技术具有深远的影响。随着技术的不断改进，我们可以期待未来会有更多突破性的发现和进展，这一切都要归功于transfection这一基本过程。