acridine orange

简明释义

吖啶橙

英英释义

例句

1.The staining protocol required us to incubate the samples with acridine orange for 30 minutes.

染色程序要求我们将样品与丙啶橙孵育30分钟。

2.In our laboratory, we use acridine orange to stain DNA in order to visualize the cells under a fluorescence microscope.

在我们的实验室中，我们使用丙啶橙来染色DNA，以便在荧光显微镜下观察细胞。

3.Using acridine orange, we observed that the apoptotic cells showed bright green fluorescence.

使用丙啶橙，我们观察到凋亡细胞发出明亮的绿色荧光。

4.The research team found that acridine orange can effectively differentiate between live and dead cells.

研究团队发现丙啶橙可以有效区分活细胞和死细胞。

5.For the experiment, we added acridine orange to the cell culture to assess the viability of the cells.

为了实验，我们向细胞培养物中添加了丙啶橙以评估细胞的活力。

作文

Acridine orange is a vital fluorescent dye used in various biological and medical applications. This compound is particularly significant in the field of microscopy, where it serves as a powerful tool for staining nucleic acids in cells. The unique properties of acridine orange allow researchers to visualize DNA and RNA within different cellular structures, making it an essential component in many laboratory protocols. By binding to nucleic acids, acridine orange emits bright fluorescence when exposed to specific wavelengths of light, enabling scientists to observe cellular activity with remarkable clarity.The versatility of acridine orange extends beyond simple staining; it also plays a critical role in distinguishing between live and dead cells. When used in conjunction with other dyes, such as propidium iodide, acridine orange can help identify viable cells by staining live cells green, while dead cells appear red. This ability to differentiate between cell viability is crucial in various research areas, including cancer studies, microbiology, and toxicology.Moreover, acridine orange has applications in the diagnosis of certain diseases. For instance, it can be utilized in detecting viral infections by staining infected cells, allowing for better visualization of pathogen presence. Medical professionals can employ this technique to assess the severity of infections and monitor treatment efficacy.In addition to its diagnostic uses, acridine orange is also instrumental in studying cellular processes such as apoptosis, or programmed cell death. Researchers often use this dye to investigate the mechanisms behind cell death, which is fundamental in understanding various diseases, including cancer. By observing how cells respond to treatments and the subsequent changes in fluorescence, scientists can glean insights into the effectiveness of therapeutic interventions.The application of acridine orange is not limited to laboratory settings; it also holds potential in clinical environments. For example, during surgeries, this dye can be used to highlight tumor margins, assisting surgeons in achieving complete resection of cancerous tissues. The ability to visualize these margins in real-time significantly improves surgical outcomes and minimizes the risk of leaving behind malignant cells.Despite its numerous advantages, working with acridine orange requires caution. The dye is known to be mutagenic, which means it can cause genetic mutations in living organisms. Therefore, appropriate safety measures must be taken when handling this compound to ensure the well-being of researchers and patients alike.In conclusion, acridine orange is an invaluable tool in the realms of biology and medicine. Its ability to stain nucleic acids, differentiate between live and dead cells, and assist in disease diagnosis makes it a staple in laboratories worldwide. As research continues to advance, the applications of acridine orange are likely to expand, offering new insights into cellular functions and disease mechanisms. Understanding the significance of this dye not only enhances our knowledge of cellular biology but also paves the way for innovative medical practices that can improve patient care and treatment outcomes.

丙啶橙是一种重要的荧光染料，广泛应用于生物学和医学领域。该化合物在显微镜学中尤为重要，作为一种强有力的工具，用于染色细胞中的核酸。丙啶橙的独特性质使研究人员能够在不同的细胞结构中可视化DNA和RNA，使其成为许多实验室协议中的基本组成部分。通过与核酸结合，丙啶橙在暴露于特定波长的光时会发出明亮的荧光，使科学家能够以惊人的清晰度观察细胞活动。丙啶橙的多功能性不仅限于简单的染色；它在区分活细胞和死细胞方面也发挥着关键作用。当与其他染料（如碘化丙啶）结合使用时，丙啶橙可以通过将活细胞染成绿色而使死细胞呈红色来帮助识别存活的细胞。这种区分细胞活性的能力在癌症研究、微生物学和毒理学等多个研究领域至关重要。此外，丙啶橙还在某些疾病的诊断中发挥着重要作用。例如，它可以用于检测病毒感染，通过染色受感染的细胞，允许更好地可视化病原体的存在。医疗专业人员可以利用这种技术评估感染的严重程度并监测治疗效果。除了其诊断用途外，丙啶橙在研究细胞过程（如凋亡或程序性细胞死亡）方面也具有重要意义。研究人员经常使用这种染料来研究细胞死亡背后的机制，这对理解包括癌症在内的各种疾病至关重要。通过观察细胞对治疗的反应以及随之而来的荧光变化，科学家们可以深入了解治疗干预的有效性。丙啶橙的应用不仅限于实验室环境；它在临床环境中也具有潜力。例如，在手术过程中，这种染料可以用于突出肿瘤边缘，帮助外科医生完全切除癌性组织。实时可视化这些边缘的能力显著改善了手术结果，并最大限度地降低了留下恶性细胞的风险。尽管有许多优点，但使用丙啶橙时需要谨慎。已知该染料具有致突变性，这意味着它可能导致生物体内的基因突变。因此，在处理这种化合物时，必须采取适当的安全措施，以确保研究人员和患者的健康。总之，丙啶橙是生物学和医学领域中不可或缺的工具。其染色核酸、区分活细胞和死细胞、以及协助疾病诊断的能力使其成为全球实验室的基础。随着研究的不断进展，丙啶橙的应用可能会扩展，为细胞功能和疾病机制提供新的见解。理解这种染料的重要性不仅增强了我们对细胞生物学的认识，还为创新医疗实践铺平了道路，以改善患者护理和治疗效果。