ultraviolet spectrophotometry

简明释义

紫外线分光光度测定法

英英释义

例句

1.The ultraviolet spectrophotometry 紫外分光光度法 technique is essential for determining the purity of pharmaceutical compounds.

该ultraviolet spectrophotometry 紫外分光光度法技术对确定药物化合物的纯度至关重要。

2.Using ultraviolet spectrophotometry 紫外分光光度法, we can detect trace amounts of pollutants in water samples.

通过使用ultraviolet spectrophotometry 紫外分光光度法，我们可以检测水样中的微量污染物。

3.In the laboratory, we use ultraviolet spectrophotometry 紫外分光光度法 to analyze the concentration of DNA samples.

在实验室中，我们使用ultraviolet spectrophotometry 紫外分光光度法来分析DNA样本的浓度。

4.The ultraviolet spectrophotometry 紫外分光光度法 method is widely used in environmental monitoring.

该ultraviolet spectrophotometry 紫外分光光度法方法广泛应用于环境监测。

5.Researchers often rely on ultraviolet spectrophotometry 紫外分光光度法 to measure protein concentrations in biological samples.

研究人员通常依赖ultraviolet spectrophotometry 紫外分光光度法来测量生物样本中的蛋白质浓度。

作文

Ultraviolet light is a form of electromagnetic radiation that has a wavelength shorter than visible light but longer than X-rays. One of the most important techniques in analytical chemistry that utilizes this type of radiation is ultraviolet spectrophotometry (紫外分光光度法). This method allows scientists to measure the absorbance or transmission of ultraviolet light by a sample, providing valuable information about its chemical composition and concentration. In this essay, I will discuss the principles of ultraviolet spectrophotometry and its various applications in different fields.The basic principle of ultraviolet spectrophotometry involves passing ultraviolet light through a sample and measuring the amount of light that is absorbed by the sample at specific wavelengths. When a molecule absorbs UV light, it undergoes a transition to a higher energy state. The extent of this absorption is directly related to the concentration of the absorbing species in the sample, according to Beer-Lambert law. This relationship allows for quantitative analysis of substances in a solution, making ultraviolet spectrophotometry an essential tool in laboratories.One of the primary applications of ultraviolet spectrophotometry is in the field of pharmaceuticals. In drug development and quality control, it is crucial to determine the concentration of active pharmaceutical ingredients (APIs) in formulations. By using ultraviolet spectrophotometry, researchers can quickly and accurately assess the purity and concentration of drugs, ensuring they meet regulatory standards. This technique is also employed in the analysis of biological samples, such as blood or urine, to monitor drug levels and detect potential overdoses.Another significant application of ultraviolet spectrophotometry is in environmental monitoring. Water quality testing often involves measuring the concentration of pollutants, such as heavy metals and organic compounds, which can be detected using this method. For instance, the presence of certain pesticides in water sources can be quantified using ultraviolet spectrophotometry, helping to ensure safe drinking water and protect aquatic ecosystems. Additionally, this technique is utilized in air quality assessments, where it can measure the concentration of harmful gases and particulate matter.In the field of biochemistry, ultraviolet spectrophotometry plays a crucial role in studying nucleic acids and proteins. DNA and RNA absorb UV light at specific wavelengths, allowing scientists to quantify these biomolecules in a sample. This is particularly important in molecular biology research, where understanding the concentration of nucleic acids is vital for various applications, including cloning and sequencing. Similarly, proteins can be analyzed using ultraviolet spectrophotometry, as their aromatic amino acids absorb UV light. This enables researchers to determine protein concentration and monitor changes in protein structure under different conditions.In conclusion, ultraviolet spectrophotometry (紫外分光光度法) is a powerful analytical technique that has widespread applications across various fields, including pharmaceuticals, environmental science, and biochemistry. Its ability to provide rapid and accurate measurements of absorbance makes it an invaluable tool for researchers and professionals alike. As technology continues to advance, the capabilities of ultraviolet spectrophotometry are likely to expand, further enhancing its role in scientific research and industry.